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protein chip  (R&D Systems)


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    Structured Review

    R&D Systems protein chip
    Protein Chip, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+chip+array/Proteome+Profiler+Rat+XL+Cytokine+Array/pmc10272649-104-7-16
    Average 95 stars, based on 57 article reviews
    protein chip - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Modified black phosphorus quantum dots promotes spinal cord injury repair by targeting the AKT signaling pathway
    Article Snippet: .. The protein solution was incubated with the protein chip (Proteome Profiler Rat XL Cytokine Array, ARY030, R D systems, MN, USA) according to the manufacturer’s instructions. .. Finally, samples were detected using a chemiluminescence imaging analysis system (ImageQuant Las4000mini, Molecular Dynamics, CS, USA).

    Article Title: Exploring the dual role of Mangifera indica L. in regulating immune response and pain persistence in inflammatory bowel disease.
    Article Snippet: The resulting data matrix was imported into the PLS toolbox version 8.6.1 (Eigenvector Research, Manson, Washington, USA) within the MATLAB environment, normalized using Norm1, autoscaled, and subjected to principal component analysis (PCA). .. : ARY020) protein arrays (both from R&D System), equal volumes (1.5 ml) of pulled colon tissues homogenates from all experimental conditions (on Day 7 and Day 21) were incubated with the pre-coated proteome profiler array membranes according to the manufacturer's instructions. .. Dot plots were visualized using an enhanced chemiluminescence detection kit and Image Quant 400 GE Healthcare software (GE Healthcare, Milan, Italy) and the resulting data were quantified using GS 800 imaging densitometer software (Biorad, Segrate, Italy) as previously described [15].

    other:

    Article Title: Tobacco Smoke Condensate Induces Morphologic Changes in Human Papillomavirus-Positive Cervical Epithelial Cells Consistent with Epithelial to Mesenchymal Transition (EMT) with Activation of Receptor Tyrosine Kinases and Regulation of TGFB
    Article Snippet: Protein extractions obtained from vehicle- and CSC-treated cells at 72 h were used to conduct RTK phospho-protein array analysis (R&D Systems, CAT# ARY001B, Minneapolis, MN, USA), with each array containing 49 specific antibodies for human RTKs.

    Recombinant:

    Article Title: PlexinD1 is a driver and a therapeutic target in advanced prostate cancer
    Article Snippet: U0126, cyclopamine, and GANT61 were purchased from Selleck Chemicals. .. An ErbB3 neutralizing antibody and human recombinant Sema3E, Sema3C, PlexinD1, and ErbB3 proteins were purchased from R&D Systems. .. Tucatinib and trastuzumab were purchased from MedChemExpress.

    Expressing:

    Article Title: Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration
    Article Snippet: .. Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E). ..

    Fluorescence:

    Article Title: Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration
    Article Snippet: .. Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E). ..

    Imaging:

    Article Title: Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration
    Article Snippet: .. Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E). ..

    Western Blot:

    Article Title: Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration
    Article Snippet: .. Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E). ..

    Stable Transfection:

    Article Title: Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration
    Article Snippet: .. Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E). ..

    Microarray:

    Article Title: Amphiphilic cytokine traps remodel marrow adipose tissue for hematopoietic microenvironment amelioration
    Article Snippet: .. Changes, especially browning, in the expression of proteins about adipocyte metabolism in marrow induced by AC-gelatin composites. (A) Fluorescence colocalization imaging of the UCP1 + adipocytes through represented by UCP1 and Plin (white triangles); all scale bars are 200 μm. (B) MAT browning promotion by AC-gelatin at 5 weeks in statistics of Fig. A. (C) Protein expression tests of osteo-organoid and bone marrow by Western blotting; β-tubulin, a protein expressed stably in adipocytes, was used as the internal reference. (D) Protein expression tests of whole marrow by protein microarray (Proteome Profiler Mouse Adipokine Array Kit, R&D Systems) and the quantified data (E). ..

    Protein Array:

    Article Title: Methods to enhance microvascular engraftment of bioengineered and primary tissues
    Article Snippet: Images were analyzed with an ApoTome.2 Optical sectioning system (Carl Zeiss). .. Human Cytokine Protein Array The presence of selected cytokines was evaluated in samples of CM with human cytokine protein arrays (R&D Systems Inc.) according to the manufacturer's instructions. .. Antigen-antibody complexes were visualized using LumiGLO substrate (Kirkegaard & Perry Laboratories, Inc.) and chemiluminescent sensitive film (Kodak).



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    Bio-Rad seldi protein chip arrays
    Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization <t>time-of-flight</t> <t>(SELDI-TOF)</t> mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.
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    Image Search Results


    Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.

    Journal: Breast Cancer Research : BCR

    Article Title: Novel serum protein biomarker panel revealed by mass spectrometry and its prognostic value in breast cancer

    doi: 10.1186/bcr3676

    Figure Lengend Snippet: Identification of serum proteins by immunodepletion and western blotting. Proteins representing peaks at m/z 6624, m/z 8916 and m/z 13870 were immunodepleted from serum extracts using monoclonal antibodies against (A) apolipoprotein CI (ApoCI), (B) C3a/ C3a des-arginine anaphylatoxin (C3a-desArg) or (C) transthyretin. The starting material (Starting, upper panel), the immunodepleted sample (Depleted, middle panel) and the eluted fraction (Recovered, lower panel) were analyzed on NP20 arrays by surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry (MS). (D) Western blotting for ApoCI, C3a/C3a-desArg and transthyretin were performed on four healthy volunteer (HV) samples and four breast cancer patient (BC) samples. (E) Mean band densities (+standard deviation (SD)) derived from the blots shown in panel D (n = 4 for all groups). (F-H) Association between SELDI peak intensities and western blotting band densities for individual HV and BC serum samples (n = 8), indicating strong correlations for (F) ApoCI, (G) C3a-desArg and (H) transthyretin.

    Article Snippet: All serum samples were initially denatured in buffer containing 8 M urea, 1% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfate) and analyzed by TOF MS on SELDI protein chip arrays (Bio-Rad, Hercules, CA, USA) as previously described [ ].

    Techniques: Western Blot, Mass Spectrometry, Standard Deviation, Derivative Assay